How is the enzyme catalase affected by changes in temperature and pH?

The general role of enzymes on living things is to speed up the chemical reactions that the body needs to function right. Enzymes function by bringing substrates together to form new molecules. They can break a substate apart to form two or more products at the end. They can also alter a substrate to make a different molecule. The specific fuction of a caltalase is to break downs molecules like hydrogen peroxide. This experiment shows if the liver enzymes becomes denatured. How will the cold liver be affected by the acid, base, water and H2O2? How will the boiled liver be affected by the acid, base, water and H2O2? The iv's of this experiment are the H2O2, acid, base, and the water. The DV of this experiment is the cold, boiled and room temperture liver. I think the cold liver will have more of a reactioun to the peroxide. The acid will increase the reaciton and the base will decrease the reaction. The hot will havea decreased reaction to the H2O2.

For the temperture study follow these instructions. Place two pieces of liver in the bottom of three different test tubes. Have cold liver, boiled liver, and room temperture liver. Add 5 mL of hydrogen peroxde to te liver in each tube and record the realtive amount of bubbline in each tube you see.

For the pH study follow these instructions. Place 2 small pieces or room temperture liver in three different test the tubes Acid, Base, and H2O. Then add 5 mL of the acid to a tube of liver. Then add 5 mL of hydrogen peroxide to the solution. Repeat step four and five for the base and the water.

My hypothesis was supported by my H2O2 had more of an effect on the cold liver. The acid will increase the reaction was not supported. The water increased the reaction. The base did decrease the reaction it acutally denatured it. The boiled liver had a decreased reaction. When you make the enzyme pH higher or lower from it's relative pH level you denature it which causes it to not funtion right. A strong acid or a base denatures it because it's causing the normal pH level to change and change it's shape. Denature means to change shape. Tempertures over 50 degress Celcious change the shape of the enzymes. Room temperture enzymes work better because they are at their natural pH level. If it's at 0 degrees celcious then it's too cold for the enzyme to function right.

Three errors in this experiment would be the room temperture was colder than normal. Anther error would be the time the liver was mixed with the acid, base and water and the difference between the times the peroxide was added. The scoring system was kind of off. Some results were supported by the results.